Effect of type 1 diabetic mellitus on the opening of myocardial mitochondrial permeability transition pore in rats
- VernacularTitle:1型糖尿病对大鼠心肌细胞线粒体通透性转换孔开放的影响
- Author:
Dan SONG
;
Xunmin CHENG
;
Shisen JIANG
;
Xiang TANG
- Publication Type:Journal Article
- Keywords:
diabetic mellitus,type 1;
myocardium;
reactive oxygen species;
membrane potential,mitochondrial
- From:
Medical Journal of Chinese People's Liberation Army
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of diabetic mellitus on the opening of mycardial mitochondrial permeability transition pore (MPTP) in rats,and to explore the role of the opening of MPTP in the development of diabetic cardiomyopathy.Methods Sixteen male Wistar rats were randomly divided into normal control group (NC) and diabetic mellitus group (DM) (8 each).Diabetic mellitus model was reproduced by a single intraperitoneal injection of streptozotocin (STZ).After 12 weeks,the production rate of mitochondrial reactive oxygen species (ROS) and the change in mitochondrial membrane potential (MMP) in myocardial mitochondria were assayed by fluorospectrophotometer.Cardiomyocyte apoptosis was evaluated by terminal-deoxynucleotidyl transferase mediated deoxy-UTP nick end labeling (TUNEL),and the glutathione (GSH) level in serum was measured by spectrophotometer.Results Compared with NC group [13.01?3.31U/(s?mg)],the production rate of mitochondrial ROS was significantly increased in DM group [28.79?6.11U/(s?mg),P
