TLR4 and MD2 antisense genes suppress activation of alveolar typeⅡ epithelial cells induced by lipopolysaccharide
- VernacularTitle:TLR4及MD2反义基因对内毒素诱导的肺泡Ⅱ型细胞活化的影响
- Author:
Yongwang LI
;
Li MA
;
Deming ZHANG
;
Guisheng QIAN
- Publication Type:Journal Article
- Keywords:
lipopolysaccharides;
pulmonary alveoli;
epithelial cells;
cells,cultured;
receptors,cell surface;
signal transduction;
Toll-like receptor 4;
myeloid differentiation protein-2
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of the antisense genes of Toll-like receptor 4 (TLR4 ) and myeloid differentiation protein-2 (MD2) on the activation of NF-?B in alveolar typeⅡepithelial cells (ATⅡcells) induced by lipopolysaccharide (LPS). Methods Cultured ATⅡcells were randomized to normal cells (control) group,LPS group,LPS+empty vector group,LPS+TLR4 antisense gene group,LPS+MD2 antisense gene group,LPS+TLR4-MD2 antisense gene group. The expressions of TLR4 and MD2 mRNA and protein in ATⅡcells were detected by Northern blotting and Western blotting respectively. The activity of NF-?B in ATⅡcells was measured with electrophoretic mobility shift assay (EMSA). The TNF-? and IL-6 concentrations in the supernatant of cultured ATⅡcells were tested with ELISA.Results Compared with control groups,the expressions of TLR4 and MD2 mRNA and protein,the NF-?? activity,the levels of TNF-? and IL-6 were increased remarkably in LPS group and LPS+empty vector group (P
