Activation of adenosine receptor A2a up-regulates epithelial sodium channel ?-subunit in cultured alveolar epithelium cells
- VernacularTitle:腺苷A2a受体对肺泡上皮细胞钠离子通道?亚基的上调作用
- Author:
Wang DENG
;
Changyi LI
;
Daoxin WANG
- Publication Type:Journal Article
- Keywords:
receptor,adenosine A2a;
epithelial sodium channel ?-subunit;
respiratory distress syndrome,adult
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of adenosine receptor A2a on the expression of epithelial sodium channel ?-subunit (?-ENaC) in alveolar epithelium A549 cells and the effects of adenosine receptor A2a in acute lung injury/aute respiratory distress syndrome. Methods After alveolar epithelium A549 Cells were incubated with 0,0.1,1,10 and 100 ?mol/L adenosine receptor A2a agonist CGS-21680 for 8 h or with 100 ?mol/L CGS-21680 for 0,1,4,8,24 and 48 h respectively,the mRNA and protein levels of ?-ENaC were determined by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis respectively. Results After A549 cells were incubated with CGS-21680 at different doses for 8 h,the mRNA and protein levels of ?-ENaC were elevated significantly at 0.1 ?mol/L CGS-21680 treatment compared with the control group (P
