Simvastatin prevents and treats ischemic-type biliary lesion in rats after liver transplantation
- VernacularTitle:辛伐他汀对大鼠移植肝胆管损伤的防治作用
- Author:
Weiwei JIANG
;
Shuguang WANG
;
Geng CHEN
;
Yujun ZHANG
- Publication Type:Journal Article
- Keywords:
liver transplantation;
biliary tract diseases;
bile acids and salts;
simvastatin;
phospholipid;
multidrug resistance 2
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the preventive effects of simvastatin on ischemic-type biliary lesion (ITBL) after liver transplantation. Methods Totally 90 female SD rats,6 to 8 weeks old,weighing 200 to 250 g,were randomly divided into 3 groups,sham-operation group (n=10),control group (n=40,including 20 donors and 20 receipts),and experimental group (n=40,including 20 donors and 20 receipts). Rat liver transplantation was established by reconstructing hepatic artery. The rats of experimental group were fed with a normal chow containing 0.1% simvastatin for 1 week before operation and for 2 weeks after operation. The donor liver was stored in the University of Wisconsin solution at 4 ℃ for 12 h followed by transplantation. The animals were killed in 2 weeks after operation and the bile,bile duct and liver tissue were taken. High performance liquid chromatography (HPLC) was used to detect the concentrations of bile salts. Biochemistry,histopathology,immunohistochemistry and RT-PCR were employed to measure the level of phospholipids C,observe the pathological changes of liver,detect the protein and mRNA expressions of phospholipid transfer protein (Mdr2 P-glycoprotein). Results The biliary phospholipid composition of rats in the experimental group (3.874 8?1.414 8 mg/dl) was significantly increased,compared with the sham-operation group (1.982 1?0.413 5 mg/dl) and the control group (1.428 5?0.538 7 mg/dl) (P
