Effect of thymosin ?1 on percentage of T-lymphocyte subsets in in vitro peripheral blood mononuclear cells from gastric cancer patients
- VernacularTitle:胸腺肽?1体外刺激胃癌患者外周血单个核细胞对淋巴细胞亚群影响
- Author:
Xia YANG
;
Feng QIAN
;
Kaijun LIU
;
Haiyang HE
;
Yuanzhi LAN
;
Yi TIAN
;
Xiaolan FU
;
Jian LI
;
Ji ZHANG
;
Zigang SHEN
;
Jintao LI
;
Yuzhang WU
- Publication Type:Journal Article
- Keywords:
thymosin;
monocytes;
stomach neoplasms;
lymphocyte subsets;
Th1 cells;
Th2 cells
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of thymosin ?1 (T?1) on cellular immune function in gastric cancer patients through observing its treatment on the differentiation of T-lymphocyte subsets from screened peripheral blood mononuclear cells (PBMCs). Methods PBMCs were obtained by centrifugation of blood samples from 18 healthy subjects and 32 patients with gastric cancer,and then cultured in the presence of culture medium with addition of T?1 at 50,10 and 1 ?g/ml for 2 d. T lymphocyte subsets (such as CD4+,CD8+ and CD4+ CD25+ Foxp3+ T cells) and Th1/Th2 multiplex cytokines were detected by flow cytometry (FCM). Results After PBMCs isolated from healthy people and patients were incubated with or without T?1,there was no significant change in percentage of CD4+,CD8+ peripheral lymphocyte subsets and ratio of CD4+/CD8+. There was no obvious change in the percentage of CD4+ CD25+ Foxp3+ T lymphocyte subsets in the normal control,but a significant increase was observed in the cells from patients with gastric cancer after treatment (P
