RNAi silencing of XIAP gene induces apoptosis and susceptibility to chemotherapy of osteosarcoma cell line MG63
- VernacularTitle:RNAi抑制XIAP基因诱导MG63细胞凋亡、化疗增敏及其分子机制
- Author:
Pan HE
;
Shan LIANG
- Publication Type:Journal Article
- Keywords:
RNA;
genes,X-linked;
osteosarcoma;
apoptosis;
cell cycle;
drug therapy
- From:Journal of Third Military Medical University
2002;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect RNAi-mediated inhibition of X-linked inhibit apoptosis protein (XIAP) gene on apoptosis and susceptibility to chemical therapy drugs in osteosarcoma cells and its underlying mechanism.Methods shRNA plasmid of XIAP gene were constructed. MG63 cells were conventionally cultured and divided into untreated group (group A,non-transfection),blank plasmid group (group B,psiRNA-Con transfected),the experimental group (group C,psiRNA-XIAP transfected),and 2 chemical drug interfered groups,non-transfection+0.3 ?mol Dox group (A+Dox group) and psiRNA-XIAP transfection +0.3 ?mol Dox group (C+Dox group). The XIAP expression in MG63 cells was detected by Western blotting. The proliferation of above-mentioned MG63 cells was examined by MTT assay. Flow cytometry (FCM) was employed to detect the cell apoptosis,cell cycle and Caspase-9 expression. Results Group C had a significantly lower protein level of XIAP and MTT value than group A and B (P
