CLUSTERING ANALYSIS ON FEMORAL GENE EXPRESSION PROFILES IN MICE FED HIGH-FAT DIET
- VernacularTitle:高脂日粮改变小鼠股骨基因表达谱的聚类分析
- Author:
Ying XIAO
;
Jue CUI
;
Yonghui SHI
;
Jin SUN
;
Guowei LE
- Publication Type:Journal Article
- Keywords:
gene expression profile;
clustering analysis;
bone mineral density;
high-fat diet
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes in femoral gene expression profiles in C57BL/6 mice fed high-fat diet (HFD) via clustering analysis of DNA microarray.Method Sixteen male C57BL/6 mice (4w old) were randomly assigned to two groups,8 in each,after 4-d ordinary diet for adaptation.The control group was fed with an ordinary diet,and the HFD group with HFD(19.5% lard).All mice were sacrificed at the end of 12w and the femoral gene expression was detected by oligonucleotide microarray analysis with Affymetrix Gene Chip Mouse U430A.DAVID,an online tool,was used for clustering analysis on femoral gene expression.Results Longtime administration of HFD caused femoral gene expressed differences related to cation ion channel,transcription regulation and signal transduction,bone mineralization,phosphate metabolic process regulation,and collagen synthesis.Conclusion Longtime intake of HFD will change the expression of numerous bone metabolism-related genes in bone of mice,and then inhibit bone formation.
