EFFECT OF CALCIUM PYRUVATE ON FATTY ACID METABOLISM IN RATS
- VernacularTitle:丙酮酸钙对大鼠脂肪酸代谢的影响
- Author:
Danying ZHANG
;
Quan ZHANG
;
Xiangzhen SHEN
;
Jue CHEN
- Publication Type:Journal Article
- Keywords:
calcium pyruvate;
blood lipid;
rat;
fatty acid;
acetyl-CoA carboxylase (ACC)
- From:
Acta Nutrimenta Sinica
1956;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of calcium pyruvate supplementation on body weight gain and fatty acid metabolism in rats.Method Thirty-two 150 g male SD rats were randomly divided into four groups:control group,low,middle and high dose calcium pyruvate group (0,100,500 and 1000mg/(kg.d)).Calcium pyruvate was administered intragastrically to rats-for 4 w,and then the rats were killed and blood and tissue samples were collected.Serum lipids and blood glucose were determined by biochemistry analyzer.Analysis of fatty acid composition of adipose tissue,liver tissue and musculature was determined by capillary gas chromatography and mRNA expressions of ACC-? in liver by RT-PCR.Result At the end of the experiment,the body weight and food conversion efficiency of rats in all experiment groups were significantly lower than that of control group.TG of high and middle dose group were obviously lower than that of control group.TC and blood glucose concentration were not statistically different among different groups.contents of C16:0,c9-C18:1,c9,c12-C18:2,?-C18:3 and C20:4 in the adipose tissue were significantly decreased in the experiment groups,but the content of C18:0 was not statistically different on fatty acid composition of musculature in rats.Fatty acid composition of liver tissue was remarkably lower than that of the control group,except C18:0 and c12-C18:2.mRNA expression level of ACC in liver of high dose group was significantly decreased (P
