Inhibition of Tetramethylpyrazine on the proliferation of rat airway smooth muscle cells
- VernacularTitle:川芎嗪抑制大鼠气道平滑肌细胞增殖的作用
- Author:
Yuejun QU
;
Hongbo BAI
;
Chaozhi WANG
;
Jide XU
;
Tingting ZHANG
;
Zhiyuan HAN
- Publication Type:Journal Article
- Keywords:
Tetramethylpyrazine;
ASMCs;
proliferation;
ERK1/2;
rat;
asthma
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Tetramethylpyrazine (TMP) on the proliferation of airway smooth muscle cells (ASMCs). Methods Primary ASMCs of rats were cultured. The absorbance (A490) value of ASMCs treatment with platelet-derived growth factor (PDGF) in the presence of TMP was detected by MTTto observe the anti-proliferation of TMP. The levels of ERK1/2 and p-ERK1/2 proteins were determined by Western blot.Results In presence of the TMP with different concentrations (12.5,25,50,100 and 200 ?mol?L-1) at 6,12,24,36 and 48 hours,compared with control groups,the average inhibitory rates of cell proliferation in all groups were increased significantly (P
