The research of lymphocyte proliferation using CFSE and FCM
- VernacularTitle:CFSE示踪与流式细胞仪检测法研究环磷酰胺对T淋巴细胞增殖的影响
- Author:
Jingjing BAO
;
Haixia LIN
;
Jing MA
- Publication Type:Journal Article
- Keywords:
CFSE;
quantitative analysis;
mathematical fitting;
FCM;
lymphocyte proliferation;
cyclophosphamide
- From:
Chinese Pharmacological Bulletin
1986;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To quantitatively analyze the lymphocyte proliferation using the time-series data of carboxyfluorescein diacetate succinimidyl ester(CFSE).Methods The model of immune suppression in mice was created by cyclophosphamide and CFSE dye was used for staining the lymphocyte of both control group and treatment group animals. The data were analyzed through mathematical model-fitting.Results The first generation of cell proliferation of the control group and treatment group were 27.17 h and 22.88 h; cell death rates in each division were respectively 20% and 40%; the half-life of cells before the proliferation was respectively 31.53 h and 43.32 h.Conclusions The mathematical fitting of CFSE data can quantitatively analyze the mechanism of the drugs attecting the proliferation of lymphocyte.In this experiment the mechanism of cyclophosphamide inhibiting lymphocyte proliferation may be due to the increase of cell death rate of each division caused by cyclophosphamide.
