Effect of Astragalus Membranaceus and Angelica Sinensis on plasminogen activator inhibitor-1 in cultured endothelial cells
- VernacularTitle:黄芪、当归对血管内皮细胞纤溶酶原激活物抑制剂-1的影响
- Author:
Changchun YANG
;
Zengchun MA
- Publication Type:Journal Article
- Keywords:
Astragalus Membranaceus( Ast) ,Angelica Sinensis( Ang) ,Plasminogen activator inhibitor-1( PAI-1)
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Astragalus Membranaceus( Ast) and Angelica Sinensis( Ang) on antithrombosis and explore the molecular mechanism. Methods Plasminogen activator inhibitor-1 ( PAI-1) expression and activity were selected to observe the antithrombosis effect of Ast and Ang ( 3 and 6 mg/ml) on 0. 5 ng/ml TGF-?1 treated endothelial cells. PAI-1mRNA expression was detected by RT-PCR,PAI-1 antigen assay was detected by specific enzyme-linked immunosorbent assays( ELISA) ,and PAI-1 activity was measured by amidolytical assay. Results Ast and Ang significantly inhibited PAI-1 mRNA expreesion,antigen and activity in endothelial cells. The more powerful effects could be observed when treated by Ast and Ang together. Conclusion The PAI-1 mRNA expression,antigen and activity is inhibited effectively by Ast and Ang,which may be the mechanism of their treatment for antithrombosis.
