Role of parathyroid hormone-related peptide in transdifferentiation of NRK-52E cells induced by high glucose
- VernacularTitle:甲状旁腺激素相关肽在高糖诱导NRK-52E细胞转分化中的作用
- Author:
Huasheng LIANG
;
Yuhua ZHONG
- Publication Type:Journal Article
- Keywords:
parathyroid hormone-related peptide;
cell transdifferentiation;
NRK-52E;
glucose;
in vitro
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of parathyroid hormone-related peptide ( PTHrP) in transdif-ferentiation of NRK-52E cells induced by high glucose. Methods Expression of PTHrP and its receptor 1 in NRK-52E cells incubated with 0 ( control group) ,5 ( normal glucose group) and 16. 7mmol/L glucose ( high glucose group) ,respectively,was detected by RT-PCR and Western blotting,respectively. Expression of TGF-?1,MMP2,type Ⅰ collagen and ?-SMA in NRK-52E cells of normal and high glucose groups was detected by Western blotting and ROS was detected by CM2H2DCFDA after 72 h. Results The expression levels of PTHrP,PTHrP receptor 1 ( PTH1R) ,and PTHrP mRNA were higher in high glucose group than in control and normal glucose groups ( P
