Effect of benzo ( a) pyrene on estrogen metabolism-related genes in endometrial cells
- VernacularTitle:苯并(a)芘对子宫内膜细胞中雌激素代谢相关基因的影响
- Author:
Wei ZHANG
;
Jie YAO
;
Ning GAO
- Publication Type:Journal Article
- Keywords:
benzo( a) pyrene;
CYP1A1;
endometrial cancer
- From:Journal of Third Military Medical University
2003;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the molecular mechanism of benzo( a) pyrene underlying the role of estrogen metabolism-related genes in human endometrial cancer cells. Methods Effect of benzo( a) pyrene on CYP450 was studied by exposing human endometrial cancer cells ( RL95-2) to benzo( a) pyrene at various concentrations ( 0. 016,0. 8,0. 4,2. 0 and 10 ?mol/L BP) for 12 h,or to 2 ?mol/L BP for 6,12,24,36 and 48 h. RL95-2 cells were harvested and cleaved. Expressions of CYP1A1,CYP1B1 and CYP3A4 proteins in RL95-2 supernatant were detected by Western blotting. Supersome in expressed CYP450 was used as a positive control to confirm the reliability of assay methods. Results The expression of CYP1A1 protein was significantly increased in RL95-2 cells 12 h after they were exposed to different concentrations of benzo( a) pyrene or to 2 ?mol/L BP for different periods of time,and reached it peak at 48 h,indicating that expression of CYP1A1 protein depends on the used dose and time of benzo( a) pyrene. Conclusion Benzo( a) pyrene can increase the expression of CYP1A1 in human endometrial cancer cells,cause changes in estrogen metabolism,and inhibit proliferation of human endometrial cancer ells.
