Effects of ginsenoside Rb1 on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblast cultured in vitro
- VernacularTitle:人参皂苷Rb1对大鼠成骨细胞增殖、分化及OPG/RANKL mRNA表达的影响
- Author:
Junjun WANG
;
Yangzhen JIANG
;
Jichao LIANG
;
Yong CHEN
- Publication Type:Journal Article
- Keywords:
Ginsenoside Rb1;
Osteoblast;
Proliferation;
Differentiation;
Expression of mRNA
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of ginsenoside Rb1 on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblast cultured in vitro.Methods:Rat osteoblast cultured in vitro was treated by a series of different concentration of ginsenoside Rb1 for different time respectively.The proliferation of osteoblast was detected by MTT method.The differentiation of osteoblast was detected by the activity of ALP.mRNA expression of osteoprotegerin (OPG) and RANKL(receptor activator nuclear factor-kappa B ligand,RANK) were semi-quantified by RT-PCR,and the ratio of OPG/RANKL mRNA was used to analyze the differentiation of ginsenoside Rb1 to osteoclast.Results:Ginsenoside Rb1 at a concentration of 1.12?10-9mol/L to 1.12?10-5mol/L can promote the proliferation of osteoblast after 72h of treatment (P
