Effect of puerarin on the expression of NF-?B65 and TNF-? in kidney of diabetic rats
- VernacularTitle:葛根素对糖尿病大鼠肾脏NF-?B_(65)、TNF-?表达的影响
- Author:
Xiuling CUI
;
Yuanzheng WANG
;
Xiaojian LIU
- Publication Type:Journal Article
- Keywords:
diabetes mellitus;
diabetic nephropathies drug therapy;
NF-kappaB;
tumor necrosis factor-alpha;
puerarin
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of puerarin on the expression of NF-?B65 and TNF-? in kidney of diabetic rats. Methods SD rats were randomly assigned to 5 groups (10 each):normal control group (group A),diabetic group (group B),low-dose,middle-dose and high-dose treatment groups (group C,D and E). After diabetes model was reproduced,animals in group C,D and E were i.p. injected with puerarin in a dose of 40,80 and 160mg/(kg?d),respectively; animals in group A and B were treated with corresponding normal saline. Fasting blood glucose (FBG),urinary albumin excretory rate (UAER),serum creatinine (Scr) and blood urea nitrogen (BUN) were determined at the 8th weekend after treatment. The histological changes in renal cortex were observed with light microscope and electron microscope. The qualitative and semi-quantitative expressions of NF-?B65 and TNF-? in nephridial tissue were determined by immunohistochemical method. Results FBG,UAER,Scr and BUN were higher in group C,D and E than that in group A (P
