Proliferation effects of astragaloside on neural atem cells in vitro
- VernacularTitle:黄芪甲苷对体外神经干细胞增殖作用影响的研究
- Author:
Lijuan CHAI
;
Peiru ZHONG
;
Zhihuan ZHOU
;
Xiuyun WANG
;
Ishida TORAO
;
Yanjun ZHANG
- Publication Type:Journal Article
- Keywords:
neural stem cells;
astragaloside Ⅳ;
proliferation;
Hes1;
Hes5;
cyclin D1
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of astragaloside(AS)on the proliferation of neural stem cells(NSCs).Methods The NSCs of embryonic day(E)16 SD rat were cultured in vitro.The NSCs were identified by the immunocytochemical(ICC)staining of Nestin.The ICC staining of BrdU was adopted to characterize the proliferation of NSCs.The number of neurospheres and the ICC staining of BrdU were performed to identify their proliferation properties.Real time RT-PCR technique was used to investigate the proliferation mechanisms of the AS on the NSCs.Results Characteristic protein(Nestin)of NSCs labeling,BrdU labeling were positive showed by immunocytochemical staining.The ICC of BrdU labeling results showed that different dosages of AS could promote the proliferation of NSCs in vitro.The proliferation rate of NSCs was increased extremely significant(P
