The influence of Yupingfeng polysaccharide on T lymphocyte subgroup of adjuvant arthrits rat
- VernacularTitle:玉屏风多糖对佐剂性关节炎大鼠T细胞亚群的影响
- Author:
Yingying CHEN
;
Jun LI
;
Lei SHI
;
Jiajia WANG
;
Yan HUANG
- Publication Type:Journal Article
- Keywords:
Yupingfeng polysaccharide;
adjuvant arthritis;
T cell subgroups;
IFN-?;
IL-4;
RT-PCR
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effects of Yupingfeng polysaccharide(YPF-P)on T cells of adjuvant arthritis(AA)in rats.Methods Freund's complete adjuvant was used to induce AA in rat.Secondary paw swelling of AA rats was measured with volumeter.Peripneral blood lymphocyte proliferation response induced by concanavalin A(ConA)was examined with MTT assay.The CD4+ and CD8+ T cell in peripheral blood were detected by flow cytometer.ELISA method was applied to determine the content of factor IFN-? and radio-immunity assay of factor IL-4.RT-PCR procedures were utilized to determine the expression of IFN-? mRNA.Results 80,160 mg?kg-1 YPF-P could singnificantly inhibit the secondary paw swelling of AA rats.The suppressesion of lymphocyte proliferation of peripheral blood T cell in AA rats was reversed.After treatment by YPF-P,the number of CD4+ cell and the ratio of CD4+/CD8+ markedly decreased.The level of IFN-? in YPF-P treating group was obviously decreased in comparison with that of model control group,while the level of IL-4 was increased.The mRNA expression of IFN-? was inhibited as well.Conclusion Yupingfeng polysaccharide can regulate the turbulence of periphery abnormal T cell subgroups of adjuvant arthritis rats and can recover Th1/Th2 balance to move towards Th1,which may be one of its mechanisms of inhibiting the secondary paw swelling.
