Development and application of anti-adherent evaluation model of H pylori adherent to MKN45 cell
- VernacularTitle:幽门螺杆菌黏附MKN45细胞模型的建立与应用
- Author:
Xiaoming RUAN
;
Hongsen LI
;
Chen XU
- Publication Type:Journal Article
- Keywords:
helicobacter pylori;
adhesion;
MKN45 cell;
polysaccharide;
fluorescein labeling;
Gram staining
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the adherence of H pylori to MKN45 cell model for screening and evaluating anti-adhesive drugs.Methods H pylori suspension was incubated with FITC solution in different conditions(FITC concentration and incubation time)to optimize the labeling conditions.At the same time,Gram staining was used to determine the optimal adhesion time of H pylori to MKN45 cells.Results H pylori suspension(1?106 cuf?ml-1)incubating with FITC(5 mg?L-1)for 1 h could achieve the optimal effect for FITC-labeling H pylori.Besides,Gram staining indicated that the optimal adhesion time for H pylori to MKN45 cells was 1 h.Based on the model,the crude Aloe polysaccharide was evaluated for its anti-adhesive activity and the results showed it could significantly reduce the adherence of H pylori to MKN45 cells at the concentration of 1 g?L-1(P
