Assessment of microcirculation in a hind limb ischemia model
- VernacularTitle:下肢缺血模型中微循环功能研究方法的建立
- Author:
Wenjie CAI
;
Mingjie WANG
;
Yichun ZHU
- Publication Type:Journal Article
- Keywords:
hind limb ischemia;
microcirculation;
angiography;
animal model;
fluorescent microspheres;
rats
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To establish the standards for evaluation of microcirculation in a rat hind limb ischemia model.Methods Rats were subjected to unilateral femoral artery ligation.After 4 weeks,the regional blood flow in skeletal muscles was measured by fluorescent microsphere assay.Formation of collateral vessels was determined by microangiography.Capillary density was examined by immunostaining for CD34.Results In ischemic limbs,regional blood flow decreased significantly,some collateral vessels formed with typical "corkscrew" appearance,and histological analysis showed lower capillary density.Conclusion Quantitative assessment of microcirculation in a hind limb ischemia model is established.
