Alteration of [Ca~(2+)]_i in pulmonary artery smooth muscle of pulmonary artery hypertension rats
- VernacularTitle:肺动脉高压模型大鼠肺动脉平滑肌细胞内钙的改变
- Author:
Wei CHENG
;
Zhi LI
- Publication Type:Journal Article
- Keywords:
pulmonary artery hypertension;
ryanodine receptor;
pulmonary artery smooth muscle cell;
intracellular calcium;
agonist;
fluorescence calcium measurement technique
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of [Ca2+]i in the pulmonary artery smooth muscle cells of PAH rats induced by MCT.Methods PAH rat model was established by MCT intraperitoneal injection.The PASMCs were primarily cultured and loaded with Fura-2/AM.Effects of Ryanodine receptor agonists on intra-cellular calcium were measured by Fluorescence microscopy Results After being given 10 nmol?L-1 Ryanodine,the concentration of [Ca2+]i in control group increased by(93.31?12.41)nmol?L-1;and the concentration in PAH group increased by(141.71?13.59)nmol?L-1(P
