The metastasis suppressing ability and its mechanism of calmodulin antagonist EBB in human metastatic ovarian clear cell line ES-2
- VernacularTitle:钙调素拮抗剂EBB抑制ES-2细胞转移作用机制初探
- Author:
Rui HUANG
;
Linlin JIANG
;
Xiaoming SUN
;
Yuan ZHOU
;
Xin CHENG
;
Ming YANG
;
Yuanfu XU
;
Dongsheng XIONG
;
Caiyun WANG
;
Chunzheng YANG
;
Bing PAN
;
Huifang ZHU
- Publication Type:Journal Article
- Keywords:
calmodulin antagonist;
EBB;
ES-2 cells;
metastasis;
calcium;
tumor cells migration
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the potential suppressing effect of calmodulin antagonist EBB on metastasis-associated properties of human metastatic ovarian clear cell adenocarcinoma cell ES-2.Methods MTT assay was used to assess the growth inhibition of EBB on ES-2 cells.The invasive capacity and motility potential were determined by Transwell chamber assay and Wound assay.[Ca2+]i was observed under the laser scanning confocal microscopy.Results EBB inhibited the proliferation of ES-2 cells in vitro.The IC50 on ES-2 cells was(13.67?1.56)?mol?L-1.The invasive ability and motility potential of ES-2 cells were decreased after exposure to 3,7 and 14 ?mol?L-1 EBB respectively(P
