Study on chemical hypoxia-mimetic (CoCl_2) agent-induced inflammatory reaction in human keratinocytes
- VernacularTitle:化学性低氧模拟剂氯化钴诱导人角质形成细胞炎症反应的研究
- Author:
Chunxi LIN
;
Meifen ZHANG
;
Chuntao YANG
;
Zhanli YANG
;
Hongzhong LING
;
Jinlan MENG
;
Fanqin ZENG
;
Peixi CHEN
;
Jianqiang FENG
- Publication Type:Journal Article
- Keywords:
chemical hypoxia;
human skin keratinocytes;
interleukin 6;
interleukin 8;
inflammatory reaction;
heme oxygenase-1
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of chemical hypoxia-mimetic agent,cobalt chloride(CoCl2)on inflammatory reaction in human keratinocytes(HaCat cells).Methods After HaCat cells were treated with CoCl2 at different concentrations to set up a chemical hypoxia-induced cell model of skin injury,cell viability,intracellular reactive oxygen species(ROS),mitochondrial membrane potential(MMP),the levels of both interleukin 6(IL-6)and interleukin 8(IL-8)as well as the expression of heme oxygenase-1(HO-1)were detected.Results The viability of HaCat cells was reduced by CoCl2 at the concentrations from 500 to 3 000 ?mol?L-1,and the higher CoCl2 doses,the lower cell viability was.CoCl2 induced oxidative stress reaction(increasing ROS production and decreasing MMP).CoCl2 induced inflammatory reaction,enhancing the release of IL-6 and IL-8.CoCl2 at concentrations from 1 000 to 3 000 ?mol?L-1 upregulated HO-1 expression in HaCat cells.Conclusion CoCl2 induces not only oxidative stress,but also inflammatory reaction,increasing the release of both IL-6 and IL-8,as well as HO-1 expression.
