Effects of 17 ?-estradiol and exercise on bone PPAR? expression of hinder limb in ovariectomized rats
- VernacularTitle:17?-雌二醇和运动对去卵巢大鼠后肢骨组织PPAR?蛋白表达的影响
- Author:
Yongjie CHEN
;
Hong SHEN
;
Shumin BU
- Publication Type:Journal Article
- Keywords:
17 ?-estradiol;
exercise;
PPAR?;
ovariectomized rats;
osteoporosis;
bone marrow stem cell
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effects of 17?-estradiol(E2)and exercise on the protein expression of peroxisome proliferators activated receptor ?(PPAR?)in bone tissues and bone marrow adipocytes in ovariectomized rats.Methods Forty female Sprague-Dawley rats,aged three months,were randomly divided into the following four groups:sham-operation(Sham),ovariectomized(OVX),ovariectomized plus moderate-intensity treadmill exercise(EX,18 m?minute-1,45 min?day-1,5 uphill,4 times?week-1)and ovariectomized treated with E2(25 ?g?kg-1,3 times?week-1)group.At the end of experiment,the morphological changes of femur and tibia were observed decalcifiedly by HE staining and the protein expression of bone PPAR? was detected by Western blot.Results The number of fat vacuoles and PPAR? protein expression in OVX group was significantly higher than that of other three groups.Conclusion E2 and exercise can benefit the bone morphological structure and these effects might be partly related to the inhibition of both PPAR? protein expression in bone tissues and adipogenic differentiation of bone marrow in OVX rats.
