Effect of Serum containing Ziqi Ruangan Pill on Apoptosis Related Genes in LO2
- VernacularTitle:紫七软肝片含药血清调控人肝细胞凋亡相关基因的研究
- Author:
Yun CHEN
;
Fengrong JIANG
- Publication Type:Journal Article
- Keywords:
Ziqi Ruangan Pill;
LO2;
Serum pharmacology;
Cell apoptosis;
Gene
- From:
Journal of Medical Research
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. Methods Rabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene,casepase3 and NF-?B was detected by Real-time RT PCR and Western-Blot. Results The serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3,NF-?B and reduce the apoptosis. Conclusion Ziqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-?B.
