HBcAg-specific CD8~+T Cells Inhibit HBV Replication in vitro
- VernacularTitle:HBcAg肽特异性CD8~+T细胞抑制乙型肝炎病毒的体外实验研究
- Author:
Jishan ZHENG
;
Yude TANG
;
Changlun HE
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Specific CD8+T cells;
IFN-?;
HepG2.2.15 cells
- From:
Journal of Medical Research
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of HBcAg-specific CD8+T cells on inhibiting HBV replication in vitro,and to search the cytokine of noncytolytic mechanisms in viral clearance. Methods By the method of coculture of HepG2.2.15 cell (target cells) with HLA-A2 matched HBcAg-specific CD8+T cell clone (effector cells) at E:T ratios of 1:50,and monitoring HBV production (HBsAg,HBeAg,and HBV-DNA)in coculture supernatants at 24h,48h and 72h,the percentage of decrease in HBV replication level was observed. Furthermore,blocking experiment with neutralizing mAbs to IFN-? was performed to evaluate the effect of this cytokine. Results CD8+T clone produced high levels of IFN-?following coculture with 2.2.15 cells. HBsAg,HBeAg and HBV-DNA in coculture supernatants were significantly reduced,and the greatest effect was observed at 72h by 54.55%,50.36% and 74.55%,respectively. The reduction of HBV DNA was decreased followed by using neutralizing mAbs to IFN-?. The maximum activity of cytotoxicity of target cells was at 24h by 15.66%. Conclusion ①HBV-specific CD8+T cells inhibit HBV replication by cytolytic and noncytolytic mechanisms.②The effect of noncytolytic mechanisms is mainly mediated by IFN-?.
