Effects of Adiponectin on Cell Activity and Expression of T-cadherin in Human RPE Cells Cultured with High D-glucose
- VernacularTitle:脂联素对高糖中hRPE细胞活性及T-cad表达的影响
- Author:
Peng ZHANG
;
Xing LI
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Human retinal pigment epithelial(hRPE) cells;
T-cadherin
- From:
Journal of Medical Research
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of adiponectin on cell activity and expression of T-cadherin in human retinal pigment epithelial(hRPE) cells cultured with high glucose.Methods ①The hRPE cells were divided into three groups:normal glucose group(5.5mmol/L glucose),high glucose group (33mmol/L glucose),high glucose plus adiponectin group (33mmol/L glucose+2.5ug/ml adiponectin).48h later,the cell activity was observed by MTT assay.②After the above groups were cultured respectively for 24h,48h,72h again,the expression of T-cadherin mRNA was observed by RT-PCR. Results ①Cell activity in high glucose group significantly decreased. Cell activity adiponectin group was higher than that in the high glucose group.②With the extension of time ,the expression of T-cadherin decreased in high glucose group,but increased in adiponectin group. Conclusion Adiponectin may be protect the hRPE cells by T-cadherin.
