Effect of neurotrophic tyrosine kinase receptor B on in vitro invasiveness of malignant transformed hFOB1.19 cells
- VernacularTitle:神经营养因子酪氨酸激酶受体B对人永生化成骨细胞hFOB1.19恶性转化细胞体外侵袭力的影响
- Author:
Jing ZENG
;
Yusheng HUANG
;
Huanzi DAI
;
Qiaonan GUO
- Publication Type:Journal Article
- Keywords:
neurotrophic tyrosine kinase receptor B;
K252a;
osteosarcoma;
microfilament;
invasion;
metastasis
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of neurotrophic tyrosine kinase receptor B(TrKB) on in vitro invasiveness of malignant transformed hFOB1.19 cells and the role of TrKB in invasion and metastasis of osteosarcoma.Methods Expression of TrKB in malignant transformed hFOB1.19 cells and SaOS-2 osteosarcoma cells was detected by RT-PCR and immunofluorescence.Function of TrKB of malignant transformed hFOB1.19 cells was further studied.Malignant transformed hFOB1.19 cells were treated with specific tyrosine kinase inhibitor K252a for 24 h as a treatment group,and untreated malignant transformed hFOB1.19 cells into which DMSO was added served as a control group.Morphology of cells was observed under an inverted phase contrast microscope.Cell invasiveness was detected by Transwell assays.Microfilaments of cells were detected by actin immunofluorescence.Results The expression of TrKB was significantly higher in malignant transformed hFOB1.19 cells than in SaOS-2 osteosarcoma cells(P
