Effects of pulsed electromagnetic fields on osteogenic differentiation in demineralized bone matrix-induced human marrow stromal cells in vitro
- VernacularTitle:脉冲电磁场对脱钙骨基质诱导人骨髓干细胞成骨分化的影响
- Author:
Zhiping WANG
;
Feng LIU
;
Jianhua ZHAO
- Publication Type:Journal Article
- Keywords:
pulsed electromagnetic fields;
demineralized bone matrix;
human marrow stromal cells;
osteoblastic differentiation
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of pulsed electromagnetic fields (PEMF) on osteogenic differentiation in demineralized bone matrix (DBM)-induced human marrow stromal cells (hMSCs).Methods hMSCs were obtained from iliac crest marrow aspirates of a healthy boy aged 14.Then 1?104 hMSCs per well were plated in 8 24-well tissue culture plates,and 1?105 hMSCs per well in 2 6-well plates after they were expanded until passage 2.Those 24-well plates and 6-well plates were divided into 4 groups,cell control group (C group),cell-material group (CD group),cell-PEMF group (CP group) and cell-material-PEMF group (CDP group).CD group and CDP group were added with one DBM (size of 5 mm?5 mm?3 mm),CP and CDP groups were exposed to the PEMF (frequency of 15 Hz,intensity of 5 Gs),and hMSCs were cultivated in subculture medium with full media exchange in every 3 d.Alkaline phosphatase (ALP) activity and osteocalcin (OC) level were performed at 1,7,14 and 21 d,and alizarin red staining and counting of calcium nodules were performed at 21 d.Results Significant differences in ALP activity and OC level were observed between cell control group and the other 3 groups in 7 d after treatment (P
