Noggin induces human bone marrow-derived mesenchymal stem cells to differentiate into neural cells
- VernacularTitle:携带小鼠noggin基因的重组腺病毒载体对人骨髓间充质干细胞的神经样诱导作用
- Author:
Yuxiang TAO
;
Haiwei XU
;
Zhengqin YIN
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
noggin;
differentiate;
adenovirus
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether noggin alone can induce human bone marrow mesenchymal stem cells (MSCs) to differentiate into neural cells for the treatment of neural degenerative diseases.Methods Human bone marrow MSCs were primarily cultured and then divided into control,Ad group of empty adenovirus vector and Ad-noggin group of adenovirus vector with noggin gene.Their morphological changes were observed with fluorescence microscopy and laser confocal microscopy,and the results of 48 h,4,7,10 d were analyzed with statistical methods.Results Both the vector made human bone marrow MSCs carry green fluorescence.Ad group didn’t show any morphological changes.While for Ad-noggin group,a few neural-like cells appeared in 48 h after transfection.The number of such cells was increased,and there were some neurites around the cells after 4 d.The cells amplified greatly after 10 d.Compared to those of 7 d after transfection,the soma and dendritic diameters of Ad-noggin group were significantly increased after 10 d (P
