Expression of insulin receptor mRNA in skeletal muscle and oxidative stress change in diabetic rats at different intensity training
- VernacularTitle:不同运动强度下糖尿病大鼠骨骼肌胰岛素受体mRNA表达及氧化应激变化
- Author:
Rong QIAN
;
Yan TAN
;
Zongbao WANG
;
Hong YE
- Publication Type:Journal Article
- Keywords:
diabetes;
exercise intensity;
insulin receptor;
oxidative stress;
skeletal muscle
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of insulin receptor mRNA in skeletal muscle and the change of oxidative stress in diabetic rats at different intensity training.Methods Sprague-Dawley rats were divided into group A(control group),group B(non-exercise group),group C(low-intensity exercise group),group D(moderate-intensity exercise group) and group E(high-intensity exercise group),6 rats in each group.A diabetic rat model was induced by injecting streptozotocin(STZ)for 4 weeks after high lipid and glucose breeding.Rats were trained for 6 weeks on a treadmill.Serum glucose and insulin,insulin resistance index(HOMA-IR),activities of SOD and GSH-Px,and MDA were detected before and after training.Expression levels of InsR mRNA in skeletal muscle were measured by RT-PCR.Results The InsR receptor mRNA expression level in three exercise groups at different intensities was 0.55?0.05,0.53?0.04,and 0.41?0.05,respectively,and was significantly higher in low and moderate intensity exercise groups than in group B (P
