Fosinopril down-regulates transforming growth factor-activated kinase 1 in rats with renal tubulointerstitial fibrosis
- VernacularTitle:福辛普利对肾间质纤维化大鼠转化生长因子?激活激酶表达的下调作用
- Author:
Shouyan LU
;
Minglong LIU
;
Tianxi LIU
;
Jianhua LI
- Publication Type:Journal Article
- Keywords:
unilateral ureter obstruction;
renal interstitial fibrosis;
TGF-activated kinase 1;
fosinopril
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of fosinopril on the expression of transforming growth factor (TGF)-activated kinase 1 (TAK-1) in the kidney of rats with unilateral ureteral obstruction (UUO),and its protective effect on renal interstitial fibrosis (RIF).Methods UUO model was induced by ligating the left ureter in rats.A total of 72 male Sprague-Wistar rats were randomly divided into 3 groups:sham-operated group (n=24),UUO model group (n=24) and UUO+fosinopril group (n=24).The rats were treated with 10 mg/(kg?d) by gastric gavage in the fosinopril treated group from 2 d after the operation,and the rats were treated with the identical dose of normal saline in the other 2 groups.Eight rats of each group were sacrificed at 7,14 and 21 d after UUO.Pathological changes of the renal tissue were observed by HE and Masson staining,and the mRNA and protein expression of TAK1 was detected by in situ hybridization,real-time PCR and Western blot analysis.Results The renal interstitial damage index of UUO group was increased compared with that of the sham-operation group (P
