Effect of transforming growth factor-?1 on cell cycle,proliferation and invasive capacity of human colon cancer cells
- VernacularTitle:pEGFP-N1-TGF-?1重组质粒对结肠癌细胞增殖、细胞周期及侵袭性的影响
- Author:
Hong YU
;
Zheng JIANG
;
Pilong WANG
- Publication Type:Journal Article
- Keywords:
transforming growth factor-?1;
SW480 cells;
colon cancer;
invasion
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transforming growth factor-?1 (TGF-?1) on cell cycle,cell proliferation,and invasive capacity of human colon cancer cells of the line SW480,for its role in colon carcinogenesis and development,as well as its application in gene therapy for colon cancer with RNA interference.Methods The recombinant expressing plasmids pEGFP-N1-TGF-?1 was constructed and transfected into SW480 cells by Lipofectamine 2000.The expression of TGF-?1 mRNA and protein in the transfected SW480 cells were detected by fluorescent microscopy,RT-PCR and Western blot analysis respectively.The cell proliferation of SW480 cells was tested by MTT assay,the cell cycle was analyzed by flow cytometry,and the invasion ability of SW480 cells were investigated by Transwell-matrigel invasion chambers.Results After transfected into SW480 cells,both the mRNA and protein levels of TGF-?1 were effectively increased (P
