RNAi-mediated silencing of HIF-1? inhibits growth and metastasis of colon cancer in tumor-bearing mice
- VernacularTitle:RNA干扰缺氧诱导因子-1?对裸鼠结肠癌生长及转移的影响
- Author:
Shangkun LI
;
Zhongxue FU
;
Mingyang LI
- Publication Type:Journal Article
- Keywords:
RNA interference;
hypoxia-inducible factor 1?;
nude mice;
SW480 cells
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of RNAi-mediated hypoxia-inducible factor 1? (HIF-1?) silencing on the growth and metastasis of colon cancer in nude mice.Methods A total of 30 6-week-old female nude mice were randomly divided into normal saline group (NS group),negative plasmid group and RNA interference group after injected with SW480 subcutaneously to form a colon carcinoma.shRNA-HIF-1? vector was constructed and injected (50 ?g/time,once per 2 d,for 7 times) into the tumor mass of nude mice of RNA interference group to induce RNAi.Normal saline or blank vector was injected into the corresponding nude mice.The changes of HIF-1? were detected with RT-PCR and Western blot analysis.The size of tumors,metastasis rate and the positive rate of lymphnode were compared among different groups.Results In 15 d after RNAi vector injection,the tumor volume of RNAi group [(273.9?14.7) mm3] was much smaller than those of NS group [(1 845.5?91.2) mm3] and negative plasmid group [(1 842.7?115.7) mm3] (P
