Effect of diterpenoid C from Radix Curcumae on proliferation of human gastric cancer cells SGC-7901
- VernacularTitle:温郁金醚提物中二萜类化合物C体外对人胃癌细胞的抑制作用
- Author:
Haifeng JIN
;
Bin LV
;
Zhe CHEN
;
Zhongjun MA
- Publication Type:Journal Article
- Keywords:
Gstric carcinoma;
Radix Curcumae;
Apoptosis
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the biological effects of new monomer 3 from Radix Curcumae on proliferation of human gastric cancer cells SGC-7901.Methods:Uing fluorescence microscope to observe the impact on SGC-7901 cells with different concentrations of the new monomer 3 at different times,using MTT assay to observe the growth inhibition rate of SGC-7901 cells,using flow cytometry to observe the apoptosis rate and cell cycle distribution of SGC-7901 cell.Results:Pronounced apoptosis had been found by fluorescence microscopy,apoptosis had a certain amount of time-dependent and concentration-dependent;Early apoptotic cells near normal morphology,normal nuclear morphology;late apoptotic cells'size shrink or round in shape,nuclear pyknosis or fragmentation.MTT method showed that the new monomer 3 had significantly inhibition on SGC-7901 cells,the half inhibition rate(IC50) was 30.14?g/mL.Flow cytometry(FCM) showed that:the new monomer 3 can increase the SGC-7901 cells in the S phase and G0/G1 and reduce it in G2/M.The change had time and concentration dependence;The apoptotic rates of dosing unit(24h,48h) compared with the control group had significant differences.The maximum apoptosis rate was 60.07%.Conclusion:The new monomer 3 inhibited human gastric cancer SGC-7901 cells significantly and had the impact on cell cycle distribution of the SGC-7901 cells and induction of apoptosis.
