Effect of icariin on expression of glucose regulated protein78 in atherosclerosis tissue
- VernacularTitle:淫羊藿苷对动脉粥样硬化病灶血管平滑肌细胞葡萄糖调节蛋白78基因表达的影响
- Author:
Xiaojun SHEN
;
Yan WEI
;
Hang HE
- Publication Type:Journal Article
- Keywords:
Icariin;
Homocysteic acid;
Glucose regulated protein78;
Vascular smooth muscle cell;
Cell apoposis
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To select the differentially expressed vascular smooth muscle cell(VSMC) apoptosis gene induced by icariin,glucose regulated protein 78(GRP78),to observe the expression of GRP78 in atherosclerosis tissue and to the elucidate the mechanism of anti-atherosclerosis of icariin.Methods:The model was established by using halfcystine in viro.The vascular smooth muscle cell was incubated with various concentrations of icariin for 48 hours.The apoptosis was detected by TUNEL assay.GRP78 gene was amplificated by PCR,recombinant plasmid were constructed,transfected into E.coli,positive clonings were selected and conf irmed by restriction endonucleas analysis,and sequence-ed.Tissue situ hybridization was used to observe the GRP78 expression in atherosclerosis tissue.Results:Icariin promoted obviously vascular smooth muscle cell apoptosis.Furthermore,the fragment,648bp,was similar to the gene of GRP78 from VSMC,and the expression level was signif icantly higher in high cholesterol group than in control nroup.Conclusions:The GRP78 plays a key role in inducing atherosclerotic lesions.The mechanism of anti-atherosclerosis of icariin might be partly due to promoting apoptosis of vascular smooth muscle cell.
