Effects of Bortezomib on proliferation, cell cycle and activation of NF-?B of non-small cell lung cancer cells
- VernacularTitle:Bortezomib对非小细胞肺癌细胞系增殖、细胞周期及核转录因子活性的影响
- Author:
Mengmeng QIN
;
Qingxia FAN
- Publication Type:Journal Article
- Keywords:
carcinoma, non-small-cell lung;
bortezomib;
NF-kappa B;
genes, bcl-2
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Bortezomib on proliferation, apoptosis, cell cycles and activation of NF-?B of non-small cell lung cancer cells (NSCLC) in vitro. Methods The inhibitory action of Bortezomib on cellular growth was determined by MTT. The effects of Bortezomib on cell cycle and apoptosis were assessed by flow cytometry. The influence of Bortezomib on the expressions of NF-?B, I?B and Bcl-2 were detected with Western blotting. Results The inhibitory effects of Bortezomib on the proliferation of NSCLC cells showed a time-and concentration-dependent manner. The growth of NSCLC cells was arrested at G2/M stage after treatment with Bortesomib at 25nmol/L for 48h. Basal expression of NF-?B was found to exist in all the 6 cell lines, with NF-?B expression in nucleus showing an inverse correlation with I?B expression in cytoplasm. Bortezomib threw no significant influence on the basal expression of NF-?B, but significantly blocked the TNF-?-induced nuclear translocation of NF-?B and down-regulated the expression of anti-apoptosis protein Bcl-2 in a time-and concentration-dependent manner. Conclusion With NF-?B-dependent pathway, Bortezomib may inhibit the proliferation of NSCLC cells and induce apoptosis.
