Effect of the proteasome inhibitor MG-132 alone and combined with cisplatin on human laryngeal squamous cell carcinoma Hep-2 cell line
- VernacularTitle:蛋白酶体抑制剂MG-132及其与顺铂联合应用对喉癌Hep-2细胞株的影响
- Author:
Huiping ZHANG
;
Xiaoming LI
;
Xiuying LU
;
Li HE
- Publication Type:Journal Article
- Keywords:
laryngeal neoplasm;
carcinoma, squamous cell;
drug therapy;
proteasome inhibitor;
cisplatin
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of proteasome inhibitor MG-132 on proliferation and apoptosis of human laryngeal squamous cell carcinoma Hep-2 cell line, and to explore if cisplatin (DDP) is combined with MG-132, can enhance the cytotoxicity to laryngocarcinoma cells. Methods Hep-2 cells were treated with different doses (0, 1, 2.5, 5, 10, 20 ?mol/L) of MG-132 for 48h. In another experiment, cells were treated with 2.5?mol/L MG-132 for different length of time (0, 12, 24, 48, 72h). Cells were further divided into 4 groups: control group without any treatment, MG-132 group cells were treated with 1?mol/L MG-132 alone, DDP group were treated with 10, 20, 40 and 80?mol/L DDP alone, and MG-132+DDP group were given 1?mol/L MG-132 combined with 10, 20, 40, 80?mol/L DDP for 48h. Cell growth was assessed by methyl thiazolyl tetrazolium (MTT) assay, and apoptosis was determined with flow cytometry (FCM). Results MTT assays showed that the MG-132 can effectively inhibit the proliferation of Hep-2 cell in a dose-(r=0.944, P
