Thymosin ?1 increases proliferation and VEGF synthesis in human umbilical vein vessel endothelial cells in vitro
- VernacularTitle:胸腺肽促进人脐静脉血管内皮细胞增殖及合成VEGF的初步研究
- Author:
Shangkun TIAN
;
Xiaohong TAO
;
Hongzhou XIANG
;
Yuanhu WANG
- Publication Type:Journal Article
- Keywords:
Thymosin ?1;
human umbilical vein vessel endothelial cell;
vascular endothelial growth factor;
cell proliferation
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of thymosin ?1 on the proliferation of human umbilical veinssel endothelial cells(HUVECs) and synthesis of vascular endothelial growth factor(VEGF).Methods HUVECs(ECV-304) were cultured with the treatment of 100 ?l thymosin ?1 at 0,5 or 2.5 ?g/ml for 1 d in vitro.The proliferation of HUVECs were measured with MTT assay,cell cycle was tested with flow cytometry and the synthesis of VEGF was detected with Western blot analysis.Results Thymosinal promoted the proliferation of HUVECs,and the cells in S-phase was increased,and obviously promoted the synthesis of VEGF.Conclusion Thymosin ?1 promotes the proliferation of HUVECs and increases the synthesis of VEGF in HUVECs,and may have the effect of increasing the angiogenesis in the process of wound tissue.
