Impact of continuous veno-venous hemofiltration on endoplasmic reticulum stress and apoptosis of HUVEC cells induced by serum of patients with multiple organ dysfunction syndrome
- VernacularTitle:连续性血液滤过对多脏器功能衰竭患者血清诱导的血管内皮细胞凋亡及内质网应激的影响
- Author:
Hongwen ZHOU
;
Hua GAN
;
Xiaogang DU
;
Zhengrong LI
- Publication Type:Journal Article
- Keywords:
endoplasmic reticulum stress;
hemofiltration;
multiple organ dysfunction syndrome;
endothelial cells
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and implying mechanism of continuous veno-venous hemofiltration(CVVH) on endoplasmic reticulum stress(ERS) and apoptosis of HUVEC cells which induced by serum of patients with multiple organ dysfunction syndrome(MODS).Methods Ten patients with MODS who received CVVH and 10 healthy volunteers were involved in this study.This experiment was divided into 4 groups: healthy control,group of pre-CVVH,group of 6-hour-CVVH and group of 20-hour-CVVH.Serum of healthy volunteers and the sera of patients with MODS at pre-CVVH,6-hour-CVVH and 20-hour-CVVH were used to induced HUVEC cells respectively in vitro for 24 h.The cell apoptosis was examined by TUNEL assay,the intracellular GRP78 protein was observed by immunocytochemistry,and the mRNA expressions of GRP78,CHOP,TNF-?,and IL-6 in the cells were measured by RT-PCR.The levels of serum SOD and MDA were detected by colorimetric method,and those of of TNF-? and IL-6 of by ELISA.Results Compared with healthy control,the group of pre-CVVH showed higher cellular apoptosis rate(P
