Effect of estrogen on expressions of c-Fos and substance P in dorsal horn of spinal cord in mice following formalin stimulation
- VernacularTitle:雌激素对福尔马林致痛后小鼠脊髓背角c-Fos和P物质的影响
- Author:
Xiaohang BAO
;
Hongliang YI
;
Wenqiong GUO
;
Chengren LI
;
Xiaotang FAN
- Publication Type:Journal Article
- Keywords:
estradiol;
ovariectomy;
pain;
pain measurement;
spinal cord;
proto-oncogene proteins c-Fos;
substance P
- From:Journal of Third Military Medical University
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of estrogen on the pain score,c-Fos and substance P expressions in the dorsal horn of the spinal cord in the mice following formalin stimulation.Methods Fifteen C57/BL6 mice were randomized to 3 groups: control group(intact mice without estrogen treatment),OVX+V group(ovariectomized mice given vehicle) and OVX+E group(ovariectomized mice with subcutaneous injection of 2 ?g/d 17?-estradiol for 10 d).Pain score was used to assay the role of estrogen in affecting pain threshold in the mice following formalin injected into the right hind paw,and expressions of c-Fos and substance P in the dorsal horn of spinal cord(L3 to L5) in 2 h after injection of formalin was tested with immunohistochemisty to evaluate neuron activity and pain afferent fibers.Results Pain score was increased in ovariectomized mice following formalin stimulation,which was inhibited by estrogen especially in the early stage of secondary phase.The number of c-Fos-like immunoreactivity neuron(FLIN,P
