ANTHOCYANINS ATTENUATE LIPID ACCUMULATION THROUGH ADENOSINE MONOPHOSPHATE ACTIVATED PROTEIN KINASE IN HepG2 CELLS
- VernacularTitle:花色苷通过腺苷一磷酸激活蛋白激酶减少肝脏HepG2细胞的脂肪积聚
- Author:
Juanjuan LI
;
Min XIA
;
Wenhua LING
- Publication Type:Journal Article
- Keywords:
Anthocyanin;
AMPK;
HepG2
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of anthocyanins on hepatic lipid accumulation and to explore the possible mechanisms. Method HepG2 cells were treated with 1,10 and 100 ?mol/L cyanidin-3-0-?-glucoside (Cy-3-g) for 1h,carnitine palmitoyl transferase-I (CPT-I) protein level and acetyl CoA carboxylase (ACC) phosphorylation,triglyceride,fatty acid oxidation,the activity of AMPK,AMPK and p-AMPK protein levels were evaluated. Results Compared with the control,the treatment of HepG2 with 1,10,100 ?mol/L Cy-3-g resulted in significant decrease of intracellular triglyceride,and significant increase of CPT-I protein,and AMPK activity and phosphorylation. However,there were no differences in AMPK protein level among all groups. The treatment of 100?mol/L Cy-3-g promoted the fatty acid oxidation in HepG2. The treatment of 10,100 ?mol/L Cy-3-g significantly increased ACC phosphorylation. Conclusion Anthocyanin Cy-3-g could attenuate hepatic lipid accumulation in HepG2. The mechanism may result from the regulation of the AMPK-ACC-CPT-I signal pathway and promotion of fatty acid oxidation.
