THE STRUCTURE OF MAMMALIAN SALIVARY GLANDS AND THE STAINING PROPERTIES OF THEIR CELLS Ⅰ. PAROTID GLAND
- VernacularTitle:哺乳动物唾液腺的结构及其细胞染色性质的初步观察Ⅰ.腮腺的观察 郑镇恶
- Author:
Chene CHENG
- Publication Type:Journal Article
- From:
Acta Anatomica Sinica
1955;0(03):-
- CountryChina
- Language:Chinese
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Abstract:
The structure of the parotid glands of 22 animals, representing 7 mamma- lian orders, was briefly described and some histochemical characteristics of their cells were studied. The tissues were fixed in Helly's and Carnoy's fluids, em- bedded in paraffin and stained with McManus PAS method and Mucicarmine for mucous substance, methyl green-pyronin and gallocyanin for chromidial substance and toluidine blue for metachromasia. Millon's reagent was used for the identification of protein. In most of the material studied, the acinar cells with PAS positive granu- les were shown to be ?-metachromasia with toluidine blue. The staining reac- tions of these granules differed from that of typical-mucous cells in that the latter, although PAS positive, showed ?-instead of ?-metachromasia. In the acinar cells of rodents, men and mules, the chromidial substance was relatively abundant, while in carnivores and others it was negligible in amount. In still others mere traces were found. PAS positive substance was present on the surface or in the apical cytoplasm of the intercalary and salivary duct cells in many animals. It was not glycogen since it resisted diastase digestion. Its phy- siological signifieance awaits further elucidation. The intercalary duct of ham- ster contained PAS positive fine granules which could not be stained by muci- carmine and showed more intense Millon reaction than that of acinar cells. The salivary duct of moles had special epithelium which contained distinct spherical granules that stained pink with the PAS method.
