Effects of Resveratrol on Neonatal Rat Cardiomyocyte Apoptosis Induced by Hypoxia
- VernacularTitle:白藜芦醇对缺氧诱导大鼠乳鼠心肌细胞凋亡的作用
- Author:
Danni LIU
;
Qiulan ZHOU
;
Dan ZHU
;
Xiulan HUANG
- Publication Type:Journal Article
- Keywords:
Resveratrol;
Neonatal rat cardiomyocyte;
Hypoxia;
Apoptosis
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of resveratrol(Res) on neonatal rat cardiomyocyte lesion induced by hypoxia. Methods The cardiomyocytes of neonatal rats were cultured in vitro and the model of cardiomyocytes hypoxia was established. The cardiomyocyte vitalities were determined by MTT assay. The cardiomyocyte apoptosis was detected by Hoechst33258 fluorescent staining. The levels of total antioxidant capacity(T-AOC) and glutathione peroxidase(GSH-Px) were measured as well. Results After the administration of hypoxia for 8, 12, 16 and 24 hours, the cardiomyocyte inhibitory was(22.13?3.22)%, (29.75?0.34)%, (37.43?6.42)% and (45.47?7.32)%, respectively. After the administration of hypoxia for 24 hours, the typical morphologic changes of apoptosis in cardiomyocytes were showed.The intracellular GSH-Px activity decreased from(46.96?8.36)U/ml to(27.13?4.76)U/ml (P
