Ginsenosisde Rg3 inhibits HIF-1? and VEGF expressions in acute leukemia bone marrow stromal cells
- VernacularTitle:人参皂苷Rg3抑制急性白血病骨髓基质细胞HIF-1?及VEGF的表达及其机制探讨
- Author:
Jinliang WANG
;
Peiyan KONG
;
Wei XU
;
Dongfeng ZENG
- Publication Type:Journal Article
- Keywords:
ginsenoside Rg3;
acute leukemia;
bone marrow stromal cell;
hypoxia-inducible factor-1?;
vascular endothelial growth factor
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ginsenoside Rg3 on HIF-1? and VEGF expressions in acute leukemia bone marrow stromal cells(BMSCs)and the possible underlying mechanism.MethodsBMSCs from normal health individuals and acute leukemia patients were cultivated.The appropriate time and concentration of ginsenoside Rg3 on BMSCs' proliferation were assessed by MTT.The BMSCs were treated with ginsenoside Rg3,and mRNA levels of HIF-1? and VEGF were detected by RT-PCR.In addition,the expre-ssion of HIF-1?,VEGF,p-Akt and p-ERK proteins were measured by Western blot analysis and immunofluorescence assays.ResultsAfter the addition of ginsenoside Rg3,MTT showed that the appropriate concentration and time was 40 ?g/ml and 24 h respectively.HIF-1? and VEGF expressions at mRNA and protein levels were reduced significantly(P
