Effect of somatostatin on F-actin distribution and exocrine function in rat pancreatic acinar cells
- VernacularTitle:生长抑素对大鼠胰腺腺泡细胞内骨架蛋白F-actin分布和细胞外分泌功能的影响
- Author:
Gang HUANG
;
Weixue TANG
;
Xiaohong TAO
- Publication Type:Journal Article
- Keywords:
pancreas;
somatostatin;
cholecystokinin;
actins
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigated the effect of somatostatin(SS)on cholecystokinin-induced alterations of exocrine function and the redistribution of the F-actin in the pancreatic acinar cells.MethodsIn vitro,isolated rat pancreatic acini were divided into 12 groups:normal control group,different concentrations ofcholecystokinin-octapeptide groups(CCK-8)of 10-12,10-11,10-10,10-9 or 10-8 mol/L,SS group(10-7mol/L),SS and different dose CCK-8 groups.The acinar cells were stimulated with different concentrations of CCK-8 for 30 min in the presence or absence of 10-7 mol/L SS.Amylase and trypsin activity were measured using corresponding assay kits,and F-actin distribution in pancreatic acinar cells were detected by laser confocal microscopy.ResultsSS stabilized the structure of cytoskeleton in acinar cells,and remarkably increased the ratio of subapical/basolateral F-actin(P
