Vacuum-assisted closure up-regulates C-ski and down-regulates Smad3 in human wound tissue
- VernacularTitle:封闭负压引流技术对人慢性创面组织中C-ski及Smad3表达的影响
- Author:
Yong ZENG
;
Huagang JIAN
;
Jianchun ZONG
;
Kaixiu QIN
- Publication Type:Journal Article
- Keywords:
vacuum-assisted closure;
chronic wounds;
C-ski;
Smad3
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of vaccum-assisted closure(VAC)on the expression of C-ski and Smad3 in the human wound margin tissue and to explore their significance in triggering the wound healing.MethodsFourteen patients,9 males and 5 females with chronic wound admitted in our department from April 2007 to April 2009 were enrolled into the study after signed an informed consent,and the full-thickness skin defects were collected before treatment,and 1,4 or 7 d after treatment.Immunohistochemistry(IHC)was carried out to detect the expressions of C-ski and Smad3,and RT-PCR was used to determine their mRNA expressions of C-ski and Smad3.ResultsIHC results showed that there existed little C-ski in the wound margin tissue before treatment,and after treatment,it were gradually increased.Compared with before treatment group,the differences in 4 and 7 d after treatment were markedly significant(P0.05).Compared with above groups,the differences in 4 and 7 d after treatment groups were markedly significant(P
