Determination of Berberine Hydrochloride in San Yu Ointment by HPLC
- VernacularTitle:RP-HPLC法测定散瘀软膏中盐酸小檗碱的含量
- Author:
Hua YIN
;
Jianghua ZHANG
;
Aizhen ZOU
- Publication Type:Journal Article
- Keywords:
San Yu Ointment;
berberine hydrochloride;
RP-HPLC;
quantitative determination
- From:
Journal of Zhejiang Chinese Medical University
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To establish a content determination method of Berberine hydrochloride in San Yu Ointment by HPLC.[Methods] RP-HPLC was performed to determine Berberine hydrochloride on a column of Agilent Zorbax Eclipse XDB-C8(4.6?150mm,5?m)at 20℃; Acetonitrile-0.1 % H3PO4 (50∶50)(Adding 0.1 g C12H25NaO4S to every 100 mL)was used as mobile phase,the flow rate was 1.0 mL?min-1.The detection wavelength was at 265 nm.[Results]A good linearity can be found in the range of 39.40 ~394.0 ng,and the regression equation was Y = 4.073?103x+2.248 ? 103 (r=0.9992).The recovery was between 97.9 %~100.9 %,and the average recovery rate was 99.4 %,RSD was 1.5 %.[Conclusion]The method is simple,rapid,accurate and available,which can be used to control the quality of San Yu Ointment.
