Regulatory effect of strengthening qi and nourishing yin recipe on expressions of ER? and CyclinD1 of Lewis lung cancer in C57 mice
- VernacularTitle:益气养阴方对C57小鼠Lewis肺癌ER?及CyclinD1表达的影响
- Author:
Chunjie LI
;
Jianli SUN
;
Lingshuang LIU
;
Xiyuan SUN
- Publication Type:Journal Article
- Keywords:
TCM;
Strengthening qi and nourishing yin recipe;
Lewis lung cancer;
Er?;
CyclinD1
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of strengthening qi and nourishing yin recipe (SQNY recipe) on the expressions of estrogen receptor ? (ER?) and CyclinD1 of Lewis lung cancer in C57BL/6 mice with immunohistochemistry, and discuss the mechanism of its anti-proliferation and anti-metastasis effects, and analyse the correlation between ER? and CyclinD1. Methods: Twenty-four C57BL/6 mice were randomly divided into three groups. They were all transplanted with Lewis lung cancer cells, and received the interventions the next day as follows: tumor-bearing control group was fed with 0.9% NaCl, and TCM group was treated with SQNY recipe, and DDP group was given intraperitoneal DDP injection. All of mice were killed on the 20th day, weighed the tumor and counted lung metastases to calculate the inhibition rates of tumor growth and metastasis. The expressions of ER?, CyclinD1 in tumor tissues were detected by immunohistochemistry. Results: The inhibition rates of tumor growth and metastasis were 35.02% and 54.20% in TCM group, and were 41.18% and 39.02% in DDP group, respectively (all P
