Effect of aqueous extract of Radix et Rhizome Rhodiolae on expressions of HIF-1?, HIF-1? and VEGF in endothelial cells exposed to hypoxia
- VernacularTitle:红景天对低氧条件下内皮细胞HIF-1?、HIF-1?和VEGF表达的影响
- Author:
Xiufang GAO
;
Haiming SHI
;
Ying SHAN
;
Yong YU
;
Xuanchun WANG
- Publication Type:Journal Article
- Keywords:
Radix et Rhizome Rhodiolae;
Endothelial cells;
Hypoxia-inducible factor 1;
Vascular endothelial growth factors
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of aqueous extract of Radix et Rhizome Rhodiolae on expressions of HIF-1?, HIF-1? and VEGF in human umbilical vein endothelial cells(HUVECs) exposed to hypoxia. Methods: The effect of different treatment time and concentrations of aqueous extract of Radix et Rhizome Rhodiolae on cellular proliferation were determined with XTT colorimetric assay. The mRNA and protein expressions of HIF-1?, HIF-1? and VEGF were analyzed respectively by quantitative real-time polymerase chain reaction with SYBR Green I and Western blot. Three groups were studied: normoxia control group, hypoxia control group, Radix et Rhizome Rhodiolae treatment group. Results: The optimal condition of Radix et Rhizome Rhodiolae treatment were 24h and 10?g/mL. The protein levels of HIF-1?, HIF-1? and VEGF were elevated by hypoxia (P
