Effects of strontium on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro
- VernacularTitle:锶对人骨髓间充质干细胞增殖和成骨分化的影响
- Author:
Wei WANG
;
Jianhua ZHAO
- Publication Type:Journal Article
- Keywords:
strontium;
mesenchymal stem cells;
bone marrow cells;
cell proliferation;
cell differentiation
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of strontium (Sr) on proliferation and osteogenic differentiation of human marrow mesenchymal stem cells (hMSCs) in vitro,and to determine its optimal concentration.Methods Six milliliter of bone marrow was obtained from a healthy adult volunteer,the hMSCs were then isolated and cultured in vitro,and the cell phenotype was identified by flow cytometry.The 4th passage cells were used for experiment,and they were randomly divided into 5 groups:the osteogenic agents (10-8mol/L dexamethasone,50?g/ml ascorbic acid and 10mmol/L beta-sodium glycerophosphate) were used alone in the control group (group A),and the combination of osteogenic agents and different doses of strontium chloride (3.75?10-3,3.75?10-2,3.75?10-1 and 3.75mmol/L) were used in group B,C,D and E.The proliferation level,alkaline phosphatase (ALP) activity and osteocalcin (OCN) content of MSCs were determined by MTT colorimetric assay,enzyme-labeled method and radioimmunological method,respectively.The amount and area of calcium nodules in each group were observed by alizarin red staining.Results The proliferation and osteogenic differentiation rate of each group increased significantly with the lapse of culture time(P
